Confocal immunofluorescent analysis of p14 rat brain dentate gyrus using Phospho-CREB (Ser133) (87G3) Rabbit mAb (Alexa Fluor ® 647 Conjugate) (blue pseudocolor). Actin filaments were labeled with Alexa Fluor ® 488 Phalloidin #8878 (green). Red pseudocolor = Propidium Iodide (PI)/RNase Staining Solution #4087. Views shown are 10X magnification (left) and 60X magnification (right).
Confocal immunofluorescent analysis of SK-N-MC cells (serum-starved overnight), untreated (left) or treated with IBMX (0.5 mM) and Forskolin #3828 (30 μM, 30 min; right), using Phospho-CREB (Ser133) (87G3) Rabbit mAb (Alexa Fluor ® 647 Conjugate) (blue pseudocolor). Actin filaments were labeled with Alexa Fluor ® 488 Phalloidin #8878 (green).
Flow cytometric analysis of serum-starved SK-N-MC cells, untreated (blue) or treated with IBMX (0.5 mM) and Forskolin #3828 (30 μM, 30 min; green), using Phospho-CREB (Ser133) (87G3) Rabbit mAb (Alexa Fluor ® 647 Conjugate).