Anti-RNF21 antibody [EPR11305(2)] (ab180130) at 1/2000 dilution + Jurkat cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow cytometry analysis of K562 cells labeling RNF21 using ab180130 at 1/60 dilution. A Goat anti rabbit IgG (FITC) at 1/75 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit IgG.
Anti-RNF21 antibody [EPR11305(2)] (ab180130) at 1/10000 dilution + K562 cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution