Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab122686 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122686, at 1/200 dilution, staining ROGDI in paraffin-embedded Human liver tissue by Immunohistochemistry.
All lanes : Anti-ROGDI antibody (ab122686) at 1/250 dilutionLane 1 : Negative control (vector only transfected HEK293T lysate) Lane 2 : ROGDI over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)developed using the ECL technique