All lanes : Anti-ROGDI antibody [EPR15190] (ab184954) at 1/20000 dilutionLane 1 : Human fetal brain lysateLane 2 : Human bone marrow lysateLysates/proteins at 20 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Flow cytometric analysis of 2% paraformaldehyde-fixed HACAT cells labeling ROGDI with ab184954 at 1/50 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.