Anti-ROR alpha antibody
| Name | Anti-ROR alpha antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab60134 |
| Prices | $391.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF ChIP WB IHC-P ELISA |
| Species Reactivities | Mouse, Human, Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea Pig, Bovine, Cat, Dog, Zebrafish |
| Antigen | Synthetic peptide: GLTELHDDLS NYIDGHTPEG SKADSAVSSF YLDIQPSPDQ SGLDINGIKP, corresponding to amino acids 217-266 of Human ROR alpha(Peptide available as ab151609 |
| Blocking Peptide | ROR alpha peptide |
| Description | Rabbit Polyclonal |
| Gene | RORA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-ROR alpha antibody (ab60134) at 1.25 µg/mlLane 1 : Molecular weight markerLane 2 : Jurkat cell lysateSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling ROR alpha with ab60134 at a concentration of 4-8µg/ml. Arrows indicate positively stained epithelial cells of the renal tubule.
ICC/IF image of ab60134 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60134, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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Lu Y, Liu X, Jiao Y, Xiong X, Wang E, Wang X, Zhang Z, Zhang H, Pan L, Guan Y, Cai D, Ning G, Li X. J Clin Invest. 2014 Aug;124(8):3501-13.
The retinoid-related orphan receptor RORalpha promotes keratinocyte - The retinoid-related orphan receptor RORalpha promotes keratinocyte
Dai J, Brooks Y, Lefort K, Getsios S, Dotto GP. PLoS One. 2013 Jul 29;8(7):e70392.
The mammalian clock component PERIOD2 coordinates circadian output by interaction - The mammalian clock component PERIOD2 coordinates circadian output by interaction
Schmutz I, Ripperger JA, Baeriswyl-Aebischer S, Albrecht U. Genes Dev. 2010 Feb 15;24(4):345-57.