All lanes : Anti-RPGRIP1 antibody (ab135832) at 1/100 dilutionLane 1 : HL60 cell line lysateLane 2 : K562 cell line lysateLane 3 : MDA-MB435 cell line lysateLane 4 : Jurkat cell line lysateLysates/proteins at 35 µg per lane.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labelling RPGRIP1 with ab135832 at 1/50 dilution, followed by peroxidase-conjugated secondary antibody and DAB staining.
Flow cytometry analysis of MDA-MB231 cells labelling RPGRIP1 with ab135832 at 1/10 dilution (bottom histogram) compared to negative control cells (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.