Anti-RPL10A antibody [3G2]
| Name | Anti-RPL10A antibody [3G2] |
|---|---|
| Supplier | Abcam |
| Catalog | ab55544 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 3G2 |
| Applications | WB ICC/IF ICC/IF IHC-P FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Recombinant fragment, corresponding to amino acids 108-218 of Human RPL10A |
| Description | Mouse Monoclonal |
| Gene | RPL10A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab55484 at 10 ug/ml staining RPL10A in human Hella cells by Immunocytochemistry/ Immunofluorescence.
ab55544 at 10 ug/ml staining RPL10A in human cerebellum tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded sections).
![Overlay histogram showing HeLa cells stained with ab55544 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55544, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/24622_RPL10A-Primary-antibodies-ab55544-3.jpg)
Overlay histogram showing HeLa cells stained with ab55544 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55544, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.