Anti-RPL7 antibody

• Supplier: Abcam
• Catalog: ab72550
• Prices: $390.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB IP IHC-P
• Species Reactivities: Mouse, Human, Rat, Rabbit, Horse, Chicken, Guinea Pig, Bovine, Dog, Turkey, Pig, Monkey, Ape, Fish, Orangutan, Xenopus
• Antigen: Synthetic peptide corresponding to a region between residue 200 and the C-terminus (residue 248) of human RPL7 (NP_000962
• Description: Rabbit Polyclonal
• Gene: RPL7
• Conjugate: Unconjugated

Product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma and bronchioalveolar carcinoma pleural effusion tissue labelling RPL7 with ab72550 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.

All lanes : Anti-RPL7 antibody (ab72550) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgLane 5 : NIH3T3 whole cell lysate at 50 µg

Detection of Human RPL7 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72550 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

IHC image of ab72550 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72550, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

ICC/IF image of ab72550 stained HeLa cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72550, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

C9orf72 nucleotide repeat structures initiate molecular cascades of disease. - C9orf72 nucleotide repeat structures initiate molecular cascades of disease.

Haeusler AR, Donnelly CJ, Periz G, Simko EA, Shaw PG, Kim MS, Maragakis NJ, Troncoso JC, Pandey A, Sattler R, Rothstein JD, Wang J. Nature. 2014 Mar 13;507(7491):195-200.

Evaluation of TRAP-sequencing technology with a versatile conditional mouse - Evaluation of TRAP-sequencing technology with a versatile conditional mouse

Hupe M, Li MX, Gertow Gillner K, Adams RH, Stenman JM. Nucleic Acids Res. 2014 Jan;42(2):e14.

RRP1B targets PP1 to mammalian cell nucleoli and is associated with Pre-60S - RRP1B targets PP1 to mammalian cell nucleoli and is associated with Pre-60S

Chamousset D, De Wever V, Moorhead GB, Chen Y, Boisvert FM, Lamond AI, Trinkle-Mulcahy L. Mol Biol Cell. 2010 Dec;21(23):4212-26.

The ELAV protein HuD stimulates cap-dependent translation in a Poly(A)- and - The ELAV protein HuD stimulates cap-dependent translation in a Poly(A)- and

Fukao A, Sasano Y, Imataka H, Inoue K, Sakamoto H, Sonenberg N, Thoma C, Fujiwara T. Mol Cell. 2009 Dec 25;36(6):1007-17.