All lanes : Anti-RPL9 antibody [EP13752] (ab182556) at 1/10000 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : HCT-116 cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling RPL9 with ab182556 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Immunofluorescent analysis of 4% paraformaldehyde-fixed HCT-116 cells labeling RPL9 with ab182556 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi.
Immunofluorescent analysis of acetone-fixed HeLa cells labeling RPL9 with ab182556 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi.
Western blot analysis of HepG2 cell lysate immunoprecipitated with ab182556 at 1/50 dilution (Lane 1). Lane 2: Negative control.Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.