Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab175219 at 1/10 dilution.
Flow cytometric analysis of permeabilized HeLa cells labeling RPS12 with ab175219 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
Immunofluorescent staining of MCF7 cells labeling RPS12 with ab175219 at 1/50 dilution (green). DAPI nuclear staining (blue).
All lanes : Anti-RPS12 antibody [EPR10963] (ab175219) at 1/1000 dilutionLane 1 : Hela cell lysateLane 2 : MCF7 cell lysateLane 3 : HepG2 cell lysateLane 4 : Raji cell lysateLane 5 : C6 cell lysateLane 6 : Raw 264.7 cell lysateLane 7 : NIH-3T3 cell lysateLysates/proteins at 10 µg per lane.