Anti-RPS16 antibody
| Name | Anti-RPS16 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab26159 |
| Prices | $380.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human, Hamster, Mouse, Rat, Bovine, Dog, Pig, Zebrafish |
| Antigen | A region within a synthetic peptide: MPSKGPLQSV QVFGRKKTAT AVAHCKRGNG LIKVNGRPLE MIEPRTLQYK, corresponding to N terminal amino acids 1-50 of Human RPS16 Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | RPS16 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-RPS16 antibody (ab26159) at 1.25 µg/ml + Jurkat cell lysate
Staining of human lung tissue with ab26159 (4-8 ug/ml). Positive labelled aleveolar cells are indicated with arrows. 400X magnification.
ICC/IF image of ab26159 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26159, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-RPS16 antibody (ab26159) at 2.5 µg/mlLane 1 : Jurkat cell lysateLane 2 : Jurkat cell lysate with blocking peptide at 2 µg/mlLysates/proteins at 25 µg per lane.
Product References
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