Anti-RPS19 antibody
| Name | Anti-RPS19 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab57643 |
| Prices | $382.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Applications | WB FC |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1-146 of Human RPS19 |
| Description | Mouse Monoclonal |
| Gene | RPS19 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC-P image of RPS19 antibody (ab57643) on Kidney sections. The sections were fixed in Paraformaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in 0.3% H2O2 solution for 10 minutes at 22°C, before addition of the primary antibody at 1/100 dilution for 2 hours.See Abreview
![Overlay histogram showing HCT116 cells stained with ab57643 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57643, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/25045_RPS19-Primary-antibodies-ab57643-11.jpg)
Overlay histogram showing HCT116 cells stained with ab57643 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57643, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Active regulator of SIRT1 is required for ribosome biogenesis and function. - Active regulator of SIRT1 is required for ribosome biogenesis and function.
Knight JR, Willis AE, Milner J. Nucleic Acids Res. 2013 Apr;41(7):4185-97.
A deacetylase-deficient SIRT1 variant opposes full-length SIRT1 in regulating - A deacetylase-deficient SIRT1 variant opposes full-length SIRT1 in regulating
Shah ZH, Ahmed SU, Ford JR, Allison SJ, Knight JR, Milner J. Mol Cell Biol. 2012 Feb;32(3):704-16.
Primary hematopoietic cells from DBA patients with mutations in RPL11 and RPS19 - Primary hematopoietic cells from DBA patients with mutations in RPL11 and RPS19
Moniz H, Gastou M, Leblanc T, Hurtaud C, Cretien A, Lecluse Y, Raslova H, Larghero J, Croisille L, Faubladier M, Bluteau O, Lordier L, Tchernia G, Vainchenker W, Mohandas N, Da Costa L. Cell Death Dis. 2012 Jul 26;3:e356.
Drosha mediates destabilization of Lin28 mRNA targets. - Drosha mediates destabilization of Lin28 mRNA targets.
Qiao C, Ma J, Xu J, Xie M, Ma W, Huang Y. Cell Cycle. 2012 Oct 1;11(19):3590-8.
Phenotypic expression of maternally inherited deafness is affected by RNA - Phenotypic expression of maternally inherited deafness is affected by RNA
Bykhovskaya Y, Mengesha E, Fischel-Ghodsian N. Mol Genet Metab. 2009 Aug;97(4):297-304.