All lanes : Anti-RPS3 antibody [EPR7807] (ab181992) at 1/2000 dilutionLane 1 : HepG2 cell lineLane 2 : Raw264.7 cell lineLane 3 : NIH 3T3 cell lineLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescent analysis of 4% paraformaldehyde fixed HepG2 cell line staining RPS3 using ab181992 at a 1/500 dilution. Goat anti rabbit IgG (Alexa Fluor®555) was used as a secondary antibody, at a dilution of 1/200. Counter stain: Dapi (blue).