Flow cytometry analysis of U87-MG cells using ab181121 at a 1/290 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.
Immunofluorescence analysis of SH-SY5Y cells (fixative 4% paraformaldehyde) labeling RPUSD3 with ab181121 at a 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution.
All lanes : Anti-RPUSD3 antibody [EPR12911] (ab181121) at 1/1000 dilutionLane 1 : U87-MG lysateLane 2 : SH-SY5Y lysateLane 3 : 293T lysateLane 4 : Human fetal brain lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution