Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and mitochondria. Recommended concentration of ab122571 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122571, at 1/50 dilution, staining RPUSD4 in paraffin-embedded Human colon tissue by Immunohistochemistry.
All lanes : Anti-RPUSD4 antibody (ab122571) at 1/250 dilutionLane 1 : Vector only transfected HEK293T cell lysateLane 2 : RPUSD4 overexpression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in HEK293T cellsdeveloped using the ECL technique