All lanes : Anti-RRAS [EPR15843] antibody (ab191399) at 1/1000 dilutionLane 1 : Human fetal kidney lysateLane 2 : Human fetal heart lysateLane 3 : HT29 cell lysateLysates/proteins at 20 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Anti-RRAS [EPR15843] antibody (ab191399) at 1/10000 dilution + A549 cell lysate at 20 µgSecondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Flow cytometry analysis of paraformaldehyde-fixed A549 cells labeling RRAS with ab191399 at a 1/350 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).