All lanes : Anti-RREB1 antibody (ab98174) at 1 µg/mlLane 1 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)Lane 2 : Lung (Human) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 110 kDa (why is the actual band size different from the predicted?)Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.Exposure time : 3 minutes