Immunofluorescent analysis of 293T cells (4% paraformaldehyde-fixed) labeling RRP7A with ab185225 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
All lanes : Anti-RRP7A antibody [EPR14412] - C-terminal (ab185225) at 1/5000 dilutionLane 1 : MCF7 cell lysateLane 2 : 293T cell lysateLane 3 : A431 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Anti-RRP7A antibody [EPR14412] - C-terminal (ab185225) at 1/1000 dilution + HeLa cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution