All lanes : Anti-RSU1 [EPR16127] antibody (ab191409) at 1/1000 dilutionLane 1 : Human placenta tissue lysateLane 2 : Caco 2 cell lysateLane 3 : Jurkat cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-RSU1 [EPR16127] antibody (ab191409) at 1/10000 dilution + Caco 2 cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-RSU1 [EPR16127] antibody (ab191409) at 1/1000 dilutionLane 1 : Mouse brain tissue lysateLane 2 : Mouse heart tissue lysateLane 3 : Mouse kidney tissue lysateLane 4 : Mouse spleen tissue lysateLane 5 : Rat brain tissue lysateLane 6 : Rat heart tissue lysateLane 7 : Rat kidney tissue lysateLane 8 : Rat spleen tissue lysateLane 9 : C6 cell lysateLane 10 : Raw264.7 cell lysateLane 11 : PC12 cell lysateLane 12 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunoprecipitation analysis of Caco 2 cell lysate labeling RSU1 using ab191409 at 1/100 dilution (Lane 1). An anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of Caco 2 cell lysate.