Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099]

• Supplier: Abcam
• Catalog: ab92336
• Prices: $403.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR3099
• Applications: WB IP IHC-P ICC/IF FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to residues at the C-terminus of Human RUNX1/ AML1
• Blocking Peptide: RUNX1 / AML1 peptide
• Description: Rabbit Monoclonal
• Gene: RUNX2
• Conjugate: Unconjugated

Product images

developed using the ECL technique

All lanes : Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab92336) at 1/10000 dilutionLane 1 : MOLT4 cell lysateLane 2 : fetal thymus lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Immunohistochemistry staining of RUNX1 / AML1 in formalin-fixed, paraffin-embedded Human tonsil tissue using 1/100 ab92336.

Flow cytometric analysis of permeabilized Molt-4 cells using anti-RUNX1 ab92336 (red) or a rabbit IgG (negative) (green).

ab92336 staining RUNX1 / AML1 in human glioblastoma cell line by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0,1% Triton X 100 in PBS, blocked with 0.5% BSA for 30 minutes at room temperature and then incubated with ab92336 at a 1/50 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/400 dilution. Nuclei are counterstained with DAPI.See Abreview

ab92336 staining RUNX1 / AML1 in rat glioblastoma cell line C6 by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0,1% Triton X 100 in PBS, blocked with 0.5% BSA for 30 minutes at room temperature and then incubated with ab92336 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a Cy3 conjugated goat anti-rabbit polyclonal used at a 1/400 dilution. Nuclei are counterstained with DAPI.See Abreview

Product References

Interleukin-7 receptor mutants initiate early T cell precursor leukemia in murine - Interleukin-7 receptor mutants initiate early T cell precursor leukemia in murine

Treanor LM, Zhou S, Janke L, Churchman ML, Ma Z, Lu T, Chen SC, Mullighan CG, Sorrentino BP. J Exp Med. 2014 Apr 7;211(4):701-13.

A ThPOK-LRF transcriptional node maintains the integrity and effector potential - A ThPOK-LRF transcriptional node maintains the integrity and effector potential

Vacchio MS, Wang L, Bouladoux N, Carpenter AC, Xiong Y, Williams LC, Wohlfert E, Song KD, Belkaid Y, Love PE, Bosselut R. Nat Immunol. 2014 Oct;15(10):947-56.

PDGF receptor alpha+ mesoderm contributes to endothelial and hematopoietic cells - PDGF receptor alpha+ mesoderm contributes to endothelial and hematopoietic cells

Ding G, Tanaka Y, Hayashi M, Nishikawa S, Kataoka H. Dev Dyn. 2013 Mar;242(3):254-68.

Distinct temporal requirements for Runx1 in hematopoietic progenitors and stem - Distinct temporal requirements for Runx1 in hematopoietic progenitors and stem

Tober J, Yzaguirre AD, Piwarzyk E, Speck NA. Development. 2013 Sep;140(18):3765-76.

Differentiation of an embryonic stem cell to hemogenic endothelium by defined - Differentiation of an embryonic stem cell to hemogenic endothelium by defined

Chiang PM, Wong PC. Development. 2011 Jul;138(13):2833-43.

Online nanoflow multidimensional fractionation for high efficiency phosphopeptide - Online nanoflow multidimensional fractionation for high efficiency phosphopeptide

Ficarro SB, Zhang Y, Carrasco-Alfonso MJ, Garg B, Adelmant G, Webber JT, Luckey CJ, Marto JA. Mol Cell Proteomics. 2011 Nov;10(11):O111.011064.