Anti-RUNX2 antibody
| Name | Anti-RUNX2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab54868 |
| Prices | $394.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Applications | WB IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Rat, Human |
| Antigen | Recombinant fragment: NPRPSLNSAP SPFNPQGQSQ ITDPRQAQSS PPWSYDQSYP SYLSQMTSPS IHSTTPLSST RGTGLPAITD VPRRISDDDT ATSDFCLWPS TLSKKSQAGA , corresponding to amino acids 251-351 of Human RUNX2 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | RUNX2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-RUNX2 antibody (ab54868) at 1/1000 dilutionLane 1 : UMR-106 Rat osteosarcoma cell line, nuclear cell lysateLane 2 : UMR-106 Rat osteosarcoma cell line, cytoplasmic cell lysateSecondaryHRP conjugated goat anti-mouse antibody at 1/500 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Anti-RUNX2 antibody (ab54868) at 1 µg/ml + Cell lysate prepared from K-562 cells
ab54868 at 3 ug/ml staining RUNX2 in human placenta tissue section by Immunohistochemistry (Formalin /PFA fixed paraffin embedded tissue sections).
![Overlay histogram showing Saos 2 cells stained with ab54868 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54868, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Saos 2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/25858_RUNX2-Primary-antibodies-ab54868-11.jpg)
Overlay histogram showing Saos 2 cells stained with ab54868 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54868, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Saos 2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Parathyroid hormone and phosphorus overload in uremia: impact on cardiovascular - Parathyroid hormone and phosphorus overload in uremia: impact on cardiovascular
Custodio MR, Koike MK, Neves KR, dos Reis LM, Graciolli FG, Neves CL, Batista DG, Magalhaes AO, Hawlitschek P, Oliveira IB, Dominguez WV, Moyses RM, Jorgetti V. Nephrol Dial Transplant. 2012 Apr;27(4):1437-45.
Phosphorus overload and PTH induce aortic expression of Runx2 in experimental - Phosphorus overload and PTH induce aortic expression of Runx2 in experimental
Graciolli FG, Neves KR, dos Reis LM, Graciolli RG, Noronha IL, Moyses RM, Jorgetti V. Nephrol Dial Transplant. 2009 May;24(5):1416-21.