All lanes : Anti-RWDD1 antibody [EPR13715(B)] (ab181994) at 1/50000 dilutionLane 1 : Jurkat cell lysateLane 2 : Hela cell lysateLane 3 : 293 cell lysateLane 4 : A673 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow cytometric analysis of 2% paraformaldehyde-fixed Hela cells labeling RWDD1 with ab181994 at a 1/10 dilution, or with rabbit IgG isotype control. Goat anti rabbit IgG (FITC) at a 1/150 dilution was used as a secondary antibody.
Immunoprecipitation of Jurkat labeling RWDD1 using ab181994 at a 1/60 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as a secondary antibody at a dilution of 1/1500. Blocking/dilution buffer and concentration: 5% NFDM/TBST