Anti-S adenosylhomocysteine hydrolase antibody
| Name | Anti-S adenosylhomocysteine hydrolase antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab54733 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgM |
| Applications | WB FC ELISA |
| Species Reactivities | Human |
| Antigen | Recombinant full length (aa 1-433) Human S adenosylhomocysteine hydrolase with tag ((AAH11606) |
| Description | Mouse Monoclonal |
| Gene | AHCY |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-S adenosylhomocysteine hydrolase antibody (ab54733) at 1/500 dilution + A549 cell lysate at 25 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Anti-S adenosylhomocysteine hydrolase antibody (ab54733) at 1/500 dilution + recombinant S adenosylhomocysteine hydrolase at 0.2 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution
![Overlay histogram showing A549 cells stained with ab54733 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54733, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/25984_S-adenosylhomocysteine-hydrolase-Primary-antibodies-ab54733-1.jpg)
Overlay histogram showing A549 cells stained with ab54733 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54733, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.