Anti-S100 alpha 2 antibody [EPR5392]

• Supplier: Abcam
• Catalog: ab109494
• Prices: $313.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR5392
• Applications: WB IP IHC-P ICC/IF FC
• Species Reactivities: Human
• Antigen: A synthetic peptide corresponding to residues at the C-terminus of Human S100 alpha 2
• Description: Rabbit Monoclonal
• Gene: S100A2
• Conjugate: Unconjugated

Product images

Overlay histogram showing A431 cells stained with ab109494 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109494, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-S100 alpha 2 antibody [EPR5392] (ab109494) at 1/1000 dilutionLane 1 : HACAT cell lysateLane 2 : BxPC3 cell lysateLane 3 : A431 cell lysateLysates/proteins at 10 µg per lane.

ab109494 at 1/250 dilution staining S100 alpha 2 in paraffin-embedded Human squamous cell cervical carcinoma tissue

Immunohistochemical analysis of paraffin-embedded human skin tissue using ab109494 at 1/250 dilution.