Anti-S100 alpha 6 antibody [EPNCIR121]
| Name | Anti-S100 alpha 6 antibody [EPNCIR121] |
|---|---|
| Supplier | Abcam |
| Catalog | ab134149 |
| Prices | $387.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPNCIR121 |
| Applications | WB IP FC ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Recombinant full length Human S100 alpha 6 (P06703) |
| Description | Rabbit Monoclonal |
| Gene | S100A6 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing SHSY-5Y cells stained with ab134149 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134149, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-S100 alpha 6 antibody [EPNCIR121] (ab134149) at 1/1000 dilutionLane 1 : Fetal lung cell lysatesLane 2 : A549 cell lysatesLane 3 : Ovary cancer cell lysatesLane 4 : SH-SY5Y cell lysatesLane 5 : Human T-78 cell lysatesLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/26035_S100-alpha-6-Primary-antibodies-ab134149-1.jpg)
All lanes : Anti-S100 alpha 6 antibody [EPNCIR121] (ab134149) at 1/1000 dilutionLane 1 : Fetal lung cell lysatesLane 2 : A549 cell lysatesLane 3 : Ovary cancer cell lysatesLane 4 : SH-SY5Y cell lysatesLane 5 : Human T-78 cell lysatesLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
Immunofluorescence analysis of HeLa cells labelled with ab134149 at 1/100 dilution.
Product References
Ca2+/S100 proteins act as upstream regulators of the chaperone-associated - Ca2+/S100 proteins act as upstream regulators of the chaperone-associated
Shimamoto S, Kubota Y, Yamaguchi F, Tokumitsu H, Kobayashi R. J Biol Chem. 2013 Mar 8;288(10):7158-68.
iTRAQ-based quantitative proteomic analysis of submandibular glands from rats - iTRAQ-based quantitative proteomic analysis of submandibular glands from rats
Alves RM, Vitorino R, Padrao AI, Moreira-Goncalves D, Duarte JA, Ferreira RM, Amado F. J Biochem. 2013 Feb;153(2):209-20.