All lanes : Anti-S100 alpha 6 antibody [EPR13084] (ab181974) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : A549 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Anti-S100 alpha 6 antibody [EPR13084] (ab181974) at 1/2000 dilution + Human fetal lung lysate at 20 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling S100 alpha 6 with ab181974 at 1/50 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Immunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling S100 alpha 6 with ab181974 at 1/500 dilution, followed by Goat anti rabbit IgG (Dylight 488) at 1/200 dilution (green). Dapi staining (blue).
Immunofluorescent analysis of acetone-fixed HeLa cells labeling S100 alpha 6 with ab181974 at 1/500 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/200 dilution (red). Dapi staining (blue).