All lanes : Anti-S100 alpha 6 antibody [EPR13084-69] (ab181975) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : SH-SY5Y cell lysateLane 3 : Human fetal lung lysateLane 4 : A549 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling S100 alpha 6 with ab181975 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Immunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling S100 alpha 6 with ab181975 at 1/500 dilution, followed by Goat anti rabbit IgG (Dylight 488) at 1/200 dilution (green). Dapi staining (blue).
Immunofluorescent analysis of acetone-fixed HeLa cells labeling S100 alpha 6 with ab181975 at 1/500 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/200 dilution (red). Dapi staining (blue).
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling S100 alpha 6 with ab181975 at 1/30 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (greeen), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Western blot analysis of HeLa cell lysate immunoprecipitated with ab181975 at 1/60 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody used at 1/1000 dilution.