All lanes : Anti-S100A10 antibody [4E7E10] (ab52272) at 1/2000 dilutionLane 1 : Cell lysates prepared from MCF-7 cells Lane 2 : Cell lysates prepared from HepG2 cellsLane 3 : Cell lysates prepared from Hela cells Lysates/proteins at 100 µg per lane.SecondaryHRP-conjugated Goat polyclonal to mouse IgG
ab52272 at 1/1000 dilution staining S100A10 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). The image show positive staining in nerve and ganglion cells with ab52272.
ab52272 at 1/500 dilution staining S100A10 in human Hela (left) and L-02 (right) cells by immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG was used as secondary antibody. Image show green staining with primary antiobody whilst actin filaments were stained red with DY-554 Phalloidin. Nuclei were stained blue using DRAQ5 fluorescent DNA dye.
Overlay histogram showing HeLa cells stained with ab52272 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52272, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.