Anti-S100A10 antibody [4E7E10]
| Name | Anti-S100A10 antibody [4E7E10] |
|---|---|
| Supplier | Abcam |
| Catalog | ab52272 |
| Prices | $387.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 4E7E10 |
| Applications | FC WB ICC/IF ICC/IF IHC-P IP ELISA |
| Species Reactivities | Human |
| Antigen | Ni-NTA purified truncated recombinant S100A10 (Human) expressed in E |
| Description | Mouse Monoclonal |
| Gene | S100A10 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-S100A10 antibody [4E7E10] (ab52272) at 1/2000 dilutionLane 1 : Cell lysates prepared from MCF-7 cells Lane 2 : Cell lysates prepared from HepG2 cellsLane 3 : Cell lysates prepared from Hela cells Lysates/proteins at 100 µg per lane.SecondaryHRP-conjugated Goat polyclonal to mouse IgG](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/26156_S100A10-Primary-antibodies-ab52272-1.jpg)
All lanes : Anti-S100A10 antibody [4E7E10] (ab52272) at 1/2000 dilutionLane 1 : Cell lysates prepared from MCF-7 cells Lane 2 : Cell lysates prepared from HepG2 cellsLane 3 : Cell lysates prepared from Hela cells Lysates/proteins at 100 µg per lane.SecondaryHRP-conjugated Goat polyclonal to mouse IgG
ab52272 at 1/1000 dilution staining S100A10 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). The image show positive staining in nerve and ganglion cells with ab52272.
ab52272 at 1/500 dilution staining S100A10 in human Hela (left) and L-02 (right) cells by immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG was used as secondary antibody. Image show green staining with primary antiobody whilst actin filaments were stained red with DY-554 Phalloidin. Nuclei were stained blue using DRAQ5 fluorescent DNA dye.
![Overlay histogram showing HeLa cells stained with ab52272 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52272, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/26159_S100A10-Primary-antibodies-ab52272-4.jpg)
Overlay histogram showing HeLa cells stained with ab52272 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52272, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Interaction of calpactin light chain (S100A10/p11) and a viral NS protein is - Interaction of calpactin light chain (S100A10/p11) and a viral NS protein is
Celma CC, Roy P. J Virol. 2011 May;85(10):4783-91.
DLC1 interaction with S100A10 mediates inhibition of in vitro cell invasion and - DLC1 interaction with S100A10 mediates inhibition of in vitro cell invasion and
Yang X, Popescu NC, Zimonjic DB. Cancer Res. 2011 Apr 15;71(8):2916-25.