All lanes : Anti-S100A10 antibody [EPR3317] (ab76472) at 1/50000 dilution (purified)Lane 1 : HACAT cell lysateLane 2 : A431 cell lysateLane 3 : HT-29 cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution
All lanes : Anti-S100A10 antibody [EPR3317] (ab76472) at 1/5000 dilution (purified)Lane 1 : RAW264.7 cell lysateLane 2 : NIH/3T3 cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution
Immunohistochemical staining of paraffin embedded human squamous cell cervical carcinoma with purified ab76472 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
All lanes : Anti-S100A10 antibody [EPR3317] (ab76472) at 1/10000 dilution (unpurified)Lane 1 : HaCaT cell lysateLane 2 : A431 cell lysateLane 3 : HT-29 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/1000 dilution
Unpurified ab76472 at 1/100 dilution staining S100A10 in human colon tissue.
Unpurified ab76472 at 1/100 dilution staining S100A10 in human kidney tissue.