All lanes : Anti-S100A11 antibody [EPR11171(B)] (ab169530) at 1/1000 dilutionLane 1 : SKBR3 cell lysateLane 2 : BxPC-3 cell lysateLane 3 : PC-3 cell lysateLane 4 : HACAT cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labeled goat anti-rabbit at 1/2000 dilution
Immunofluorescent analysis of BxPC-3 cells labeling S100A11 with ab169530 at 1/250 dilution. DAPI nuclear staining (blue).
Flow cytometric analysis of permeabilized HACAT cells labeling S100A11 with ab169530 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).