Flow cytometry analysis of PC-3 cells using ab180593 at a 1/150 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.
Immunofluorescence analysis of BxPC-3 cells (fixative 4% paraformaldehyde) labeling S100A11 with ab180593 at a 1/100 dilution (left image), and counterstained with Dapi (right image). Goat anti rabbit IgG (Dylight 555) secondary used at a 1/200 diution.
Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labeling S100A11 with ab180593 at a 1/100 dilution. Prediluted ImmunoHistoprobe HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.
Anti-S100A11 antibody [EPR11172] (ab180593) at 1/20000 dilution + BxPC-3 lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-S100A11 antibody [EPR11172] (ab180593) at 1/5000 dilutionLane 1 : SKBR-3 lysateLane 2 : PC-3 lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution