Anti-S100A9 antibody [EPR3555]
| Name | Anti-S100A9 antibody [EPR3555] |
|---|---|
| Supplier | Abcam |
| Catalog | ab92507 |
| Prices | $385.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR3555 |
| Applications | WB IHC-P ICC/IF IHC-F |
| Species Reactivities | Human |
| Antigen | Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human S100A9 aa 1-100 |
| Description | Rabbit Monoclonal |
| Gene | S100A9 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-S100A9 antibody [EPR3555] (ab92507) at 1/1000 dilutionLane 1 : Breast cancer lysateLane 2 : Human tonsil lysateLane 3 : Human spleen lysateLane 4 : Peripheral blood lymphocytes lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit IgG at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/26272_S100A9-Primary-antibodies-ab92507-1.JPG)
All lanes : Anti-S100A9 antibody [EPR3555] (ab92507) at 1/1000 dilutionLane 1 : Breast cancer lysateLane 2 : Human tonsil lysateLane 3 : Human spleen lysateLane 4 : Peripheral blood lymphocytes lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit IgG at 1/2000 dilution
ab92507, at a 1/250 dilution, staining S100A9 in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.
ab92507, at a 1/250 dilution, staining S100A9 in paraffin embedded Human spleen tissue by Immunohistochemistry.
ICC/IF image of ab92507 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92507, 1/200) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgM (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Pursuing type 1 diabetes mellitus and related complications through urinary - Pursuing type 1 diabetes mellitus and related complications through urinary
Caseiro A, Barros A, Ferreira R, Padrao A, Aroso M, Quintaneiro C, Pereira A, Marinheiro R, Vitorino R, Amado F. Transl Res. 2014 Mar;163(3):188-99.
The proteomics of colorectal cancer: identification of a protein signature - The proteomics of colorectal cancer: identification of a protein signature
O'Dwyer D, Ralton LD, O'Shea A, Murray GI. PLoS One. 2011;6(11):e27718.