Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm and golgi apparatus. Recommended concentration of ab121831 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-SAMD12 antibody (ab121831) at 1/250 dilutionLane 1 : Negative control (vector only transfected HEK293T lysate) Lane 2 : Over expression Lysate (co-expressed with a C terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
ab121831, at a 1/100 dilution, staining SAMD12 in paraffin embedded Human stomach tissue by Immunohistochemistry.