All lanes : Anti-SAPK4 antibody [EPR14611] (ab188324) at 1/1000 dilutionLane 1 : THP-1 cell lysateLane 2 : 293 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
All lanes : Anti-SAPK4 antibody [EPR14611] (ab188324) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : NIH3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling SAPK4 with ab188324 at 1/50 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Immunofluorescent analysis of 4% paraformaldehyde-fixed 293 cells labeling SAPK4 with ab188324 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with DAPI.