All lanes : Anti-SAR1 antibody (ab135624) at 1/100 dilutionLane 1 : Mouse lung tissue lysateLane 2 : 293 cells lysateLysates/proteins at 35 µg per lane.
All lanes : Anti-SAR1 antibody (ab135624) at 1/100 dilutionLane 1 : Nontransfected 293 cells lysateLane 2 : Transiently transfected (with the SAR1 gene) 293 cells lysate Lysates/proteins at 2 µg per lane.
Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human lung carcinoma tissue labelling SAR1 with ab135624 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
Flow cytometric analysis of NCI-H292 cells using ab135624 at 1/10 stainning SAR1 (bottom histogram) compared to a negative control cell (top histogram). FITC conjugated goat anti-rabbit secondary antibodies were used for the analysis.