Anti-SAR1 antibody (ab77029) at 5 µg/ml + recombinant full length human SAR1 protein with a tag (immunogen) at 0.2 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilutiondeveloped using the ECL technique
Anti-SAR1 antibody (ab77029) at 5 µg/ml + Jurkat cell lysate at 25 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilutiondeveloped using the ECL technique
![Overlay histogram showing HeLa cells stained with ab77029 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77029, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/26867_SAR1-Primary-antibodies-ab77029-1.jpg)
Overlay histogram showing HeLa cells stained with ab77029 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77029, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ab77029 staining SAR1 in Panc-1 cell line from Human pancreas carcinoma by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Formaldehyde and blocked with 1% Donkey Serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/75 in PBST + 1% Serum) for 1 hour at 22°C. An Alexa Fluor®488-conjugated Donkey anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.See Abreview