Anti-SCAI antibody [EPR4128]

• Supplier: Abcam
• Catalog: ab124688
• Prices: $313.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR4128
• Applications: IHC-P ICC/IF ICC/IF WB FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide, corresponding to amino acid residues in Human SCAI
• Description: Rabbit Monoclonal
• Gene: SCAI
• Conjugate: Unconjugated

Product images

All lanes : Anti-SCAI antibody [EPR4128] (ab124688) at 1/1600 dilution (purified)Lane 1 : Mouse brain tissue lysateLane 2 : Rat brain tissue lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-SCAI antibody [EPR4128] (ab124688) at 1/1600 dilution (purified) + Human thyroid tissue lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Immunohistochemical staining of paraffin embedded human brain with purified ab124688 at a working dilution of 1 in 50. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with purified ab124688 at a dilution of 1/400. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

Overlay histogram showing SH-SY5Y cells fixed in 2% PFA and stained with purified ab124688 at a dilution of 1 in 160 (pink line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control.

All lanes : Anti-SCAI antibody [EPR4128] (ab124688) at 1/1000 dilution (unpurified)Lane 1 : Human Thymus lysateLane 2 : Human Thyroid lysateLane 3 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat-anti-rabbit HRP at 1/2000 dilution

All lanes : Anti-SCAI antibody [EPR4128] (ab124688) at 1/200 dilution (purified)Lane 1 : Mouse brain tissue lysateLane 2 : Rat brain tissue lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Immunohistochemical staining of paraffin embedded human brain with unpurified ab124688 at a working dilution of 1 in 5. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with unpurified ab124688 at a dilution of 1/50. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

Unpurified ab124688 at 1/100 dilution staining SCAI in SH-SY5Y cells by Flow cytometry (red) compared to a nonspecific negative control antibody (green).

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD