Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling SCAMP1 with ab185951 at 1/160 dilution (red) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.
All lanes : Anti-SCAMP1 antibody [EPR14493(B)] (ab185951) at 1/1000 dilutionLane 1 : U87-MG cell lysateLane 2 : HeLa cell lysateLane 3 : Neuro-2a cell lysateLane 4 : Human fetal brain cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-SCAMP1 antibody [EPR14493(B)] (ab185951) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : RAW 264.7 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution