All lanes : Anti-SCO2 antibody (ab113758) at 1 µg/mlLane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 5 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell LysateLane 6 : Caco 2 (Human colonic carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.