IHC/IF image of ab150292 stained formalin fixed paraffin embedded human normal testis. The section was incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the tissue and block non-specific protein-protein interactions. The section was then incubated with the antibody (ab150292, 0.2µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.