Anti-SCP3 antibody [Cor 10G11/7]
| Name | Anti-SCP3 antibody [Cor 10G11/7] |
|---|---|
| Supplier | Abcam |
| Catalog | ab97672 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | Cor 10G11/7 |
| Applications | IHC-P ELISA ICC/IF ICC/IF IHC-F WB |
| Species Reactivities | Mouse, Hamster, Zebrafish |
| Antigen | Hamster COR1/SCP3 protein (expressed as 6x His fusion in bacteria and purified on a Talon under denaturing conditions) |
| Description | Mouse Monoclonal |
| Gene | Sycp3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab97672 staining SCP3 in mouse embryonic day 16.5 female germ cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in Formaldehyde/SDS and permeabilized in 0.05% Tween20 and 0.05% Triton X-100 in 1xPBS prior to blocking in 10% serum for 30 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 16 hours at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated goat anti-Mouse IgG polyclonal, diluted 1/400.See Abreview
IHC-P image of SCP3 staining with ab97672 on tissue sections from adult mouse testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab97672 (1/200 dilution) for 16 hours at 4°C. The secondary was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal, used at a 1/500 dilution.See Abreview
ab97672 staining SCP3 in Mouse adult testes tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 4% paraformaldehyde and blocked with 5% BSA for 60 minutes at 25°C; antigen retrieval was by heat mediation in a 0.01M citrate buffer. Samples were incubated with primary antibody (1/200 in 1% BSA) for 1 hour at 22°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.See Abreview
Product References
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