![All lanes : Anti-Scramblase 1 antibody [1E9] (ab24923) at 0.5 µg/ml (Whole cell lysates of serum starved tumor cells were applied to SDS-PAGE and transferred to a PVDF membrane)Lane 1 : HeLaLane 2 : HepG2Lane 3 : HEK-293Lane 4 : SY5YLane 5 : JurkatLane 6 : U937Lane 7 : K562Lane 8 : THPLysates/proteins at 20000 cells per lane.SecondaryA HRP-conjugated antibodydeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/27521_ab24923_1.jpg)
All lanes : Anti-Scramblase 1 antibody [1E9] (ab24923) at 0.5 µg/ml (Whole cell lysates of serum starved tumor cells were applied to SDS-PAGE and transferred to a PVDF membrane)Lane 1 : HeLaLane 2 : HepG2Lane 3 : HEK-293Lane 4 : SY5YLane 5 : JurkatLane 6 : U937Lane 7 : K562Lane 8 : THPLysates/proteins at 20000 cells per lane.SecondaryA HRP-conjugated antibodydeveloped using the ECL technique
![Overlay histogram showing HeLa cells stained with ab24923 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24923, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/27522_Scramblase-1-Primary-antibodies-ab24923-1.jpg)
Overlay histogram showing HeLa cells stained with ab24923 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24923, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.