All lanes : Anti-Scramblase 1 antibody [EPR14249] - N-terminal (ab180518) at 1/20000 dilutionLane 1 : HeLa cell lysateLane 2 : A431 cell lysateLane 3 : 293 cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Western blot analysis of immunoprecipitation pellet from 293 lysate immunoprecipitated using ab180518 at 1/50 dilution. Secondary: Goat Anti-Rabbit IgG, (H+L)-HRP at 1/1000 dilution.
Flow cytometric analysis of A431 cells (2% paraformaldehye-fixed) labeling Scramblase 1 with ab180518 at 1/50 dilution (red) compared with a Rabbit monoclonal IgG control (green). Goat anti-rabbit IgG (FITC) secondary antibody was used at 1/75 dilution.