All lanes : Anti-SCUBE2 antibody (ab170373) at 100 Lane 1 : MDA-MB-453 cell lysateLane 2 : ZR-75-1 cell lysateLysates/proteins at 35 µg per lane.
Immunofluorescent analysis of ZR-75-1 cells labeling SCUBE2 with ab170373 at a 1/10 dilution. Primary incubation was followed by secondary antibody labeling with Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling SCUBE2 with ab170373 at a 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.