All lanes : Human SUZ12 peptide (ab12389) at 1/2000 dilutionLane 1 : Lysate of HEK293T cells transfected with pCMV6-ENTRY control cDNALane 2 : Lysate of HEK293T cells transfected with pCMV6-ENTRY SCY1 like 3 cDNALysates/proteins at 5 µg per lane.
All lanes : Anti-SCY1 like 3 antibody [4F10] (ab123897) at 1/2000 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : HT29 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.
ab123897, at 1/50 dilution, staining SCY1 like 3 in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
ab123897, at 1/100, staining SCY1 like 3 in COS7 cells transiently transfected with pCMV6-ENTRY SCY1 like 3 cDNA by Immunofluorescence.