All lanes : Anti-SDHC antibody (ab129736) at 1 µg/mlLane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell LysateLane 2 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell LysateLane 3 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear LysateLane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.