Anti-Separase antibody
| Name | Anti-Separase antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab3762 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide derived from residues 1200 - 1300 of Human Separase |
| Description | Rabbit Polyclonal |
| Gene | ESPL1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Metaphase HeLa cells were labelled with anti-ACA (anti-centromeric antigen) and anti-Separase (ab3762). ab3762 was used at a working concentratin of 1µg/ml. The arrows indicate the position of the centrosomes and demonstrate a spatial relationship between Separase and the centrosomes during metaphase. In the final panel DAPI is pseudo-coloured blue, while ACA and Separase are green and red respectively. 100x magnification.
HeLa cells were labelled with anti-ACA and anti-Separase (ab3762). ab3762 was used at a working concentration of 1µg/ml. The arrow indicates the localization of Separase at the mid-body. In the final panel DAPI is pseudo-coloured blue, while ACA and Separase are green and red respectively. 100x magnification.
ab3762 staining Separase in the Human HeLa cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with PFA, permeabilized with Triton X-100 0.5% in PBS and blocked with 10% Serum for 90 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 6 hour at 25°C. An undiluted Cy3®conjugated anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview
Product References
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The blockage of survivin and securin expression increases the cytochalasin - The blockage of survivin and securin expression increases the cytochalasin
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