Anti-Separase antibody [XJ11-1B12]
| Name | Anti-Separase antibody [XJ11-1B12] |
|---|---|
| Supplier | Abcam |
| Catalog | ab16170 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | XJ11-1B12 |
| Applications | FC WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Maltose binding protein fusion protein, corresponding to C terminal amino acids 1866-1996 of Human Separase |
| Description | Mouse Monoclonal |
| Gene | ESPL1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab16170 stained MCF7 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16170 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
![Overlay histogram showing HeLa cells stained with ab16170 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16170, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/28297_Separase-Primary-antibodies-ab16170-5.jpg)
Overlay histogram showing HeLa cells stained with ab16170 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16170, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
![Anti-Separase antibody [XJ11-1B12] (ab16170) at 10 µg/ml + MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µgSecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/28298_Separase-Primary-antibodies-ab16170-4.jpg)
Anti-Separase antibody [XJ11-1B12] (ab16170) at 10 µg/ml + MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µgSecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
Critical differences between isoforms of securin reveal mechanisms of separase - Critical differences between isoforms of securin reveal mechanisms of separase
Han X, Poon RY. Mol Cell Biol. 2013 Sep;33(17):3400-15.
Separase-dependent cleavage of pericentrin B is necessary and sufficient for - Separase-dependent cleavage of pericentrin B is necessary and sufficient for
Lee K, Rhee K. Cell Cycle. 2012 Jul 1;11(13):2476-85.
Centrosomal Aki1 and cohesin function in separase-regulated centriole - Centrosomal Aki1 and cohesin function in separase-regulated centriole
Nakamura A, Arai H, Fujita N. J Cell Biol. 2009 Nov 30;187(5):607-14.
Separase, securin and Rad21 in neural cell growth. - Separase, securin and Rad21 in neural cell growth.
Pemberton HN, Franklyn JA, Boelaert K, Chan SY, Kim DS, Kim C, Cheng SY, Kilby MD, McCabe CJ. J Cell Physiol. 2007 Oct;213(1):45-53.
Protein phosphatase 2A and separase form a complex regulated by separase - Protein phosphatase 2A and separase form a complex regulated by separase
Holland AJ, Bottger F, Stemmann O, Taylor SS. J Biol Chem. 2007 Aug 24;282(34):24623-32. Epub 2007 Jun 29.