Anti-SERCA1 ATPase antibody
| Name | Anti-SERCA1 ATPase antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab92666 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ELISA |
| Species Reactivities | Mouse, Human, Rat |
| Antigen | Synthesized peptide corresponding to an internal sequence of Human SERCA1 ATPase |
| Description | Rabbit Polyclonal |
| Gene | ATP2A1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab92666 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92666, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-SERCA1 ATPase antibody (ab92666) at 1/500 dilutionLane 1 : NIH3T3 cell lysateLane 2 : A549 cell lysateLane 3 : HT29 cell lysateLane 4 : A549 cell lysate with immunizing peptide at 10 µgLysates/proteins at 30 µg per lane.