Anti-SERCA2 ATPase antibody [EPR9392]

• Supplier: Abcam
• Catalog: ab150435
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR9392
• Applications: WB IHC-P ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human SERCA2 ATPase aa 1-100 (N terminal)
• Description: Rabbit Monoclonal
• Gene: ATP2A2
• Conjugate: Unconjugated

Product images

Overlay histogram showing HepG2 cells stained with ab150435 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab150435, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-SERCA2 ATPase antibody [EPR9392] (ab150435) at 1/20000 dilutionLane 1 : HeLa cell lysateLane 2 : A673 cell lysateLane 3 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP labelled antibody at 1/2000 dilution

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling SERCA2 ATPase with ab150435 at 1/50.

Immunohistochemical analysis of paraffin embedded Human liver tissue labelling SERCA2 ATPase with ab150435 at 1/50.

Immunofluorescence analysis of HeLa cells labelling SERCA2 ATPase with ab150435 at 1/100.